Effects of different concentrations of aluminum hydroxide adjuvant and adsorption methods on the immunogenicity of diphtheria-tetanus-acellular pertussis and inactivated poliovirus combined vaccine
10.3760/cma.j.issn.0254-5101.2018.09.008
- VernacularTitle:不同氢氧化铝佐剂和吸附方式对无细胞百白破-Sabin株脊髓灰质炎联合疫苗免疫效果的影响
- Author:
Jiangli LIANG
1
;
Qin GU
;
Na GAO
;
Yan MA
;
Qiuyan JI
;
Li SHI
;
Mingbo SUN
;
Xie HENG
Author Information
1. 中国医学科学院北京协和医学院医学生物学研究所
- Keywords:
DTaP-sIPV;
Adsorbability;
Vaccine potency;
Anti-pertussis toxin ( anti-PT) anti-body;
Anti-filamentous hemagglutinin ( anti-FHA ) antibody;
Anti-pertactin ( anti-PRN ) antibody;
Anti-diphtheria toxoid ( anti-DT) antibody;
Anti-tetanus toxoid ( anti-TT) antibody
- From:
Chinese Journal of Microbiology and Immunology
2018;38(9):697-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different concentrations and adsorption methods of aluminum hydroxide adjuvant produced by different manufacturers on the immunogenicity of the diphtheria-tetanus-acellular pertussis and inactivated poliovirus combined vaccine ( DTaP-sIPV) . Methods Five anti-gens of DTaP were adsorbed onto different concentrations (0. 42 mg/ml, 0. 47 mg/ml and 0. 52 mg/ml) of aluminum hydroxide from different manufacturers through sequential and separate adsorption. Adsorbability, anti-pertussis toxin ( PT)/filamentous hemagglutinin ( FHA)/pertactin ( PRN)/diphtheria toxoid ( DT)/tet-anus toxoid ( TT) antibodies and the potency of vaccines were detected. Results The adsorbability of alu-minum hydroxide adjuvant slightly decreased with the reduction of concentration. No significant difference in potency and antibody level was observed between sequential and separate adsorption. Moreover, no signifi-cant difference in antibody level was observed between vaccines prepared with aluminum hydroxide adjuvant produced by General Chemical Corp and our institute. Conclusion Aluminum hydroxide adjuvant produced by our institute at the concentration of 0. 52 mg/ml and separate adsorption method are suitable for prepara-tion of DTaP-sIPV.
