Genetic characteristics of CV-A12 VP1 region and clinical manifestations of CV-A12-associated se-vere hand, foot and mouth disease in Qingdao
10.3760/cma.j.issn.0254-5101.2018.09.003
- VernacularTitle:青岛市柯萨奇病毒A12型VP1区基因特征及相关重症手足口病临床表现
- Author:
Xiaoyan SHI
1
;
Yanhui ZHANG
;
Zhilei SU
;
Dan ZHAO
;
Qing CHAI
;
Jinling GONG
;
Feng ZHANG
;
Zhaoguo WANG
Author Information
1. 青岛市预防医学研究院
- Keywords:
Coxsackievirus A12;
Severe hand;
foot and mouth disease;
Phylogenetic analysis
- From:
Chinese Journal of Microbiology and Immunology
2018;38(9):658-664
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular characteristics of coxsackievirus A12 ( CV-A12) and to understand the clinical manifestations of severe hand, foot and mouth disease (HFMD) caused by CV-A12 in Qingdao. Methods Throat swabs of HFMD, herpangina and influenza-like cases from 2011 to 2016 were detected for enteroviruses ( EVs) in Qingdao. Human rhabdomyosarcoma ( RD) and human la-ryngeal carcinoma (Hep-2) cells were used for virus proliferation and CV-A12 strains were identified through a semi-nest RT-PCR. The full-length of VP1 gene of CV-A12 strains was sequenced and phylogenetically an-alyzed using MEGA7. 0 software package. Clinical data of severe HFMD cases positive for CV-A12 were col-lected and analyzed. Results CV-A12-positive HFMD, herpangina and influenza-like cases accounted for 0. 3％(18/6798), 1. 2％(2/169) and 0. 1％(1/676) in Qingdao, respectively. Most of the HFMD caused by CV-A12 in children were mild before 2013 (84. 6％, 11/13), while hospitalized severe cases with neurological symptoms (100％, 5/5) became more common after 2013. Phylogenetic analysis of the VP1 region revealed that CV-A12 strains worldwide could be divided into two genotypes, A and B. All of the CV-A12 strains successfully sequenced in Qingdao from 2011 to 2016 belonged to genotype B, and 88. 9％(16/18) of them belonged to subgenotype B2. All hospitalized severe cases of CV-A12-caused HFMD after 2013 were associated with strains in branch B2b of subgenotype B2. Conclusion CV-A12 was one of the pathogens causing HFMD, herpangina and influenza-like illness in children in Qingdao. Strains of genotype B2 were the predominant CV-A12 strains circulating in Qingdao in recent years. CV-A12-caused HFMD might complicated by nervous system damage.
