Effects of five proteins secreted by Chlamydia trachomatis on phagocytosis of mouse macrophages and dendritic cells
10.3760/cma.j.issn.0254-5101.2018.06.001
- VernacularTitle:5种沙眼衣原体分泌蛋白对小鼠巨噬细胞和树突状细胞吞噬功能的影响
- Author:
Shuping HOU
1
;
Rong XU
;
Congzhong ZHU
;
Xiaoting LI
;
Shijun SHAN
;
Huiping WANG
Author Information
1. 300052,天津医科大学总医院皮肤性病科
- Keywords:
Chlamydia trachomatis;
Secretory protein;
Macrophage;
Dendritic cell;
Phagocytosis
- From:
Chinese Journal of Microbiology and Immunology
2018;38(6):401-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effects of five proteins secreted by Chlamydia trachomatis on the phagocytosis of macrophages and dendritic cells derived from bone marrow cells of C3H/HeJ mice. Methods Glutathione S-transferase ( GST)-CT311, GST-GIgA, GST-cHtrA, GST-OmcBc and GST-Pgp3 proteins were prepared through an Escherichia coli prokaryotic expression system and purified by GST Mag-Beads. Chlamydia membrane protein GST-IncA was also prepared as a control. Proteins of interest were ob-tained by cleaving off GST-tag with PreScission protease. Macrophages (MΦ) and dendritic cells (DC) were prepared from bone marrow cells of C3H/HeJ mice and pretreated with either 100 μg/ml or 500 μg/ml of the above proteins. LPS was used as a control to testify the specificity of the proteins' functions. Four hours after pretreatment,fluorescent beads were added to culture media to evaluate the changes in phagocytosis with direct immunofluorescence assay. Results LPS and low concentration (100 μg/ml) of these proteins had no significant influence on the phagocytosis of DC and MΦ,while high concentration (500 μg/ml) of Pgp3, cHtrA and CT311 could significantly promote the phagocytosis of DC and MΦ. Conclusion Pgp3, cHtrA and CT311 can promote the in vitro phagocytosis of DC and MΦ,which may facilitate the in vivo dissemina-tion of Chlamyida trachomatis.
