Screening of aptamer binding to lipopolysaccharide of Pseudomonas aeruginosa and analysis of its inhibi-tory effect on macrophage polarization

VernacularTitle:铜绿假单胞菌表面脂多糖适配体的筛选及其抑制巨噬细胞极化的研究
Author: Ying SUN ¹ ; Wei LUO ; Xiaofang ZHANG ; Zichao JIA ; Zhidong HU ; Qian WANG
Author Information

1. 300052,津医科大学总医院检验科
Keywords: Pseudomonas aeruginosa; Lipopolysaccharide; Aptamer; Macrophage
From: Chinese Journal of Microbiology and Immunology 2018;38(5):354-360
CountryChina
Language:Chinese
Abstract: Objective To screen high-affinity aptamers binding to the surface lipopolysaccharide (LPS) of Pseudomonas aeruginosa (PA), and to analyze their inhibitory effects on macrophage polarization. Methods LPS of PA was ectracted and purified. High-affinity aptamers binding to the LPS of PA were screened by systematic evolution of ligands by exponential enrichment (SELEX). Enzyme-linked oligonucleotide assay (ELONA) and quantitative PCR (Q-PCR) were performed to investigate their influences on macrophage polarization. Results In this study, an aptamer PL-6 that could specifically bind to PA-LPS was screened suc-cessfully and found to be able to block the binding of PA-LPS to the corresponding receptor TLR4, inhibit mac-rophage M1 polarization and maintain macrophage homeostasis. Conclusion This study found a high-affinity aptamer binding to the LPS of PA, which might provide a new strategy for the prevention and treatment of sepsis caused by PA.