Monitoring of diarrheagenic Escherichia coli infections in patients in Guangdong Province during 2013 to 2016
10. 3760/cma. j. issn. 0254-5101. 2018. 05. 004
- VernacularTitle:2013—2016年广东省腹泻患者致泻性大肠埃希菌病原学监测
- Author:
Dongmei HE
1
;
Yuheng LIANG
;
Bixia KE
;
Honghui ZENG
;
Meizhen LIU
;
Hailing TAN
;
Bo-Sheng LI
;
Tong YANG
;
Changwen KE
Author Information
1. 东省疾病预防控制中心病原微生物所
- Keywords:
Diarrheagenic Escherichia coli;
Virulence gene;
Pathogen surveillance;
Antibiotic re-sistance surveillance;
Pulsed-field gel electrophoresis
- From:
Chinese Journal of Microbiology and Immunology
2018;38(5):345-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the infection status, serotype distribution, drug sensitivity and molecular characteristics of diarrheagenic Escherichia coli (DEC) in patients with diarrhea in Guangdong Province. Methods Fecal samples were collected, cultured and isolated by traditional methods. Suspected Escherichia coli isolates were confirmed by multiplex PCR used for detecting specific virulence genes and bio-chemical methods. Positive strains were serotyped, characterized for drug sensitivity and analyzed by pulsed-field gel electrophoresis ( PFGE). Results The total positive rate of DEC in patients with diarrhea was 6.26%. The positive rates of enteropathogenic Escherichia coli (EPEC), enterotoxigenic Escherichia coli (ETEC), enterohemorrhagic Escherichia coli (EHEC), enteroadherent Escherichia coli (EAEC) and en-teroinvasive Escherichia coli (EIEC) were 2. 47% , 1. 54% , 1. 32% , 0. 62% and 0. 09% , respectively, with infections primarily in children aged 0-<7 years. The total seropositive rate was 52. 54% , with EHEC accounting for 15. 00% . DEC showed high sensitivity to imipenem, ciprofloxacin, ceftazidime and cefo-taxime. The multidrug resistance rate of DEC was 58. 45% , with EPEC being the most serious for multidrug resistance. PFGE results showed that ETEC, EHEC, EPEC and EAEC had a high degree of polymorphism. Conclusion EPEC is the predominant type of DEC circulating in Guangdong Province. Third-generation cephalosporins are the first drugs of choice for treating infections in children. Ciprofloxacin can be used to treat adults. The problem of multiple drug resistance of DEC is severe and efforts to monitor DEC infections and drug resistance should be strengthened.
