Changes in follicular helper T ( Tfh) cells and Tfh-related molecules during HIV-1 infection
10. 3760/cma. j. issn. 0254-5101. 2018. 05. 001
- VernacularTitle:滤泡辅助性T细胞及其相关因子在HIV疾病进展中的变化研究
- Author:
Dan LI
1
;
Jianjun LI
;
Yafeng LI
;
Shuo WANG
;
Hua LIANG
;
Zhenzhu TANG
;
Zhiyong SHEN
;
Yueqin DENG
;
Yiming SHAO
Author Information
1. 102206 北京,中国疾病预防控制中心性病艾滋病预防控制中心,传染病预防控制国家重点实验室
- Keywords:
HIV-1;
Follicular helper T cell;
B cell
- From:
Chinese Journal of Microbiology and Immunology
2018;38(5):321-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the changes in follicular helper T (Tfh) cells during HIV-1 in-fection, to investigate the influences of Tfh cells and Tfh-related molecules on HIV-1 progression and to pro-vide references for further research on using Tfh cells in highly active antiretroviral therapy ( HAART) and vaccines. Methods This study enrolled 33 patients with HIV-1 infection, including 11 long-term nonpro-gressors (LTNP), 10 rapid progressors (RP) and 12 typical progressors (TP), and 11 healthy subjects (normal controls, NC). Peripheral blood mononuclear cells were isolated from each subject. Multicolor flow cytometry was performed to detect CD4+CD45RA-CXCR5+Tfh and CD4+CD45RA-CXCR3-CXCR5+PD-1+Tfh subsets and the levels of inducible costimulatory molecule (ICOS), IFN-γ and IL-21. Moreover, the levels of IL-10 and the percentages of CD19+B cells in plasma samples of each group were also analyzed. Relationships among Tfh, CD4 and B cells were analyzed. Results The percentages of both Tfh subsets were higher in patients with HIV-1 infection than in NC. Compared with NC, LTNP had the highest percent-age of CD4+CD45RA-CXCR3-CXCR5+PD-1+Tfh cells (P<0. 05). Expression of Tfh-related molecules ICOS, IFN-γ and IL-21 were enhanced significantly upon Staphylococcus enterotoxin B ( SEB) stimulation, ICOS+Tfh cells were negatively related with HIV-1 progression, but had a positive correlation with CD19+B cells (r=-0. 49, P<0. 01; r=0. 60, P<0. 05). IL-10 level in plasma increased significantly in patients withHIV-1 infection , especially in TP and RP ( TP vs NC : P＜0. 01 ; RP vs NC : P<0. 05 ) . Conclusion HIV-1 patients and NC had significant differences in the expression of Tfh cells and Tfh-related molecules in peripheral blood. ICOS+Tfh cells were closely related to the progression of HIV-1 infection and the function of B cells.
