Mechanism of platelet aggregation induced by Streptococcus suis serotype 2 muramidase-released pro-tein (MRP)

VernacularTitle:猪链球菌2型溶菌酶释放蛋白引起血小板聚集的机制研究
Author: Yaya PIAN ¹ ; Simei REN ; Zhenxiang GAO ; Jingjing NIE ; Ran ZHANG ; Jihong HU
Author Information

1. 100730,北京医院国家老年医学中心卫生部临床检验中心
Keywords: Streptococcus suis; Muramidase-released protein; Platelet aggregation; Integrin
From: Chinese Journal of Microbiology and Immunology 2018;38(3):211-217
CountryChina
Language:Chinese
Abstract: Objective To study the mechanism of platelet aggregation induced by Streptococcus suis serotype 2 muramidase-released protein (MRP) and to provide scientific proof and theoretical basis for clinical treatment of patients with Streptococcus suis infection. Methods Nickel column affinity chromatogra-phy was used to purify recombinant proteins of MRP-N and MRP-C. Platelet aggregometer, thromboelastog-raphy (TEG) and scanning electron microscope were used to observe the platelet aggregation induced by MRP. Results Streptococcus suis 2 wild type strain,but not the mutant strain ΔMRP,could induce platelet aggregation. It was MRP-N but not MRP-C that induced platelet aggregation. GPRP,an inhibitor of β2inte-grin receptor,could significantly inhibit the platelet aggregation induced by MRP. Conclusion Streptococ-cus suis 2 MRP induces platelet aggregation through β2integrin receptor pathway.