Cdc42-dependent endocytosis pathway in the regulation of Na+/H+exchanger 3 (NHE3) expression on rotavirus-infected Caco-2 cells
10.3760/cma.j.issn.0254-5101.2018.03.004
- VernacularTitle:Cdc42内吞途径在轮状病毒感染对Caco-2细胞NHE3蛋白调控中的作用
- Author:
Mei-Lan NIU
1
;
Peng WANG
;
Changying CHEN
;
Rongfang FENG
;
Zixiao CHEN
;
Jiawei JIAO
;
Yuanyuan LI
;
Haoyu XU
;
Ling LI
Author Information
1. 黄河科技学院药理教研室
- Keywords:
Rotavirus;
Na+/H+exchanger 3;
Cdc42-dependent endocytosis pathway
- From:
Chinese Journal of Microbiology and Immunology
2018;38(3):181-186
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects and regulatory mechanism of rotavirus infection on the expression and bioactivity of Na+/H+exchanger 3 (NHE3) on Caco-2 cells. Methods A cell model of Caco-2 cells expressing NHE3 was constructed. Four groups were set up,which were control(CTL) group, rotavirus(RV) infection group, Cdc42 inhibitor (Pirl-1) group and Pirl-1+RV group. Bioactivity and ex-pression of NHE3 on the surface of Caco-2 cells were determined by BCECF-AM and biotinylation method, respectively. Expression of Cdc42 protein was measured by Western blot. Co-immunoprecipitation was per-formed to detect the interaction between NHE3 and Cdc42. Results Compared with the CTL group,RV in-fection significantly inhibited the bioactivity and expression of NHE3 on Caco-2 cells. These inhibitory effects were antagonized by Pirl-1. Moreover,RV infection enhanced the expression of Cdc42 protein and promoted the interaction between NHE3 and Cdc42, which were also antagonized by Pirl-1. Conclusion RV infec-tion might regulate the expression and bioactivity of NHE3 through Cdc42-dependent endocytosis pathway.
