Interleukin-27:a biomarker for prediction of acute graft-versus-host disease after allogeneic hemato-poietic stem cell transplantation from unrelated donors
10.3760/cma.j.issn.0254-5101.2018.02.004
- VernacularTitle:IL-27在无关供体异基因造血干细胞移植中对移植物抗宿主病的作用
- Author:
Shuangzhu LIU
1
,
2
,
3
;
Jingjing HAN
;
Huanle GONG
;
Jiaqian QI
;
Hong LIU
;
Jia CHEN
;
Shoubao MA
;
Depei WU
Author Information
1. 215006 苏州大学附属第一医院,江苏省血液研究所,卫生部血栓与止血重点实验室
2. 215213 苏州大学造血干细胞移植研究所
3. 215006 血液学协同创新中心,江苏省干细胞与生物医用材料重点实验室,省部共建国家重点实验室培养基地
- Keywords:
Biomarker;
Graft-versus-host disease;
IL-27;
Unrelated donor transplantation
- From:
Chinese Journal of Microbiology and Immunology
2018;38(2):98-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the predictive value of serum interleukin-27 (IL-27) for acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) from unrelated donors. Methods Serum samples were collected from 72 patients after receiving allo-HSCT from unrelated donors during January to December 2012. Serum samples collected from 70 patients received allo-HSCT in 2013 were used for confirmation. All patients received myeloablative conditioning regimen prior to allo-HSCT. Cyclosporin A (CsA)+mycophenolate mofetil (MMF)+short-term methotrexate (MTX) were used for GVHD prophylaxis. Serum IL-27 levels in patients with aGVHD were measured by ELISA. The pre-dictive value of IL-27 index,defined as the ratio of serum IL-27 level at neutrophil engraftment to that before pre-conditioning regimen, for allogeneic HSCT was retrospectively analyzed. Results Serum IL-27 index was significantly decreased in patients with gradeⅡ-ⅣaGVHD(grade 0-Ⅰ : 1.89±0.68 vs gradeⅡ-Ⅳ :1.26±0.49;P<0.000 1). IL-27 index had good value for grade Ⅱ-Ⅳ aGVHD (AUC=0.782,95% CI:0.675-0.889,P<0.001). Patients with a lower serum IL-27 index (<1.33) were more likely to have a higher cumulative incidence of grade Ⅱ-Ⅳ aGVHD than those with a higher serum IL-27 index (P<0.001). Multivariate analysis confirmed that low IL-27 index was the most significant risk factor for gradeⅡ-Ⅳ aGVHD (HR=4.50,95% CI:2.1-9.8,P<0.01). These findings were consistent with the results found in the serum samples collected in 2013. Conclusion Low IL-27 index could be used to predict the incidence of grade Ⅱ-Ⅳ acute GVHD after allo-HSCT from unrelated donors.
