Follicular helper T (Tfh) cells involves in the pathogenesis of Henoch-Sch?nlein purpura through affecting CD40/CD40L axis
10.3760/cma.j.issn.0254-5101.2018.01.008
- VernacularTitle:Tfh通过CD40/CD40L轴在介导儿童过敏性紫癜发病中的作用与机制初探
- Author:
Ting LI
1
;
Xiaoxiao TANG
;
Haiping YANG
;
Mo WANG
;
Gaofu ZHANG
;
Xuelan CHEN
;
Xiaoqin LI
;
Qiu LI
Author Information
1. 400014 重庆,儿童发育疾病研究教育部重点实验室,儿科学重庆市重点实验室,儿童发育重大疾病国家国际科技合作基地
- Keywords:
Henoch-Sch?nlein purpura;
Immunoglobulin;
B cell;
Follicular helper T cell;
CD40/CD40 ligand
- From:
Chinese Journal of Microbiology and Immunology
2018;38(1):47-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether follicular helper T(Tfh) cells were involved in the development of Henoch-Sch?nlein purpura(HSP) and Henoch-Sch?nlein purpura nephritis(HSPN) in chil-dren through affecting CD40/CD40L axis. Methods Fifty-five subjects were enrolled in this study and di-vided into four groups as follows:22 children with HSP but without renal involvement(Group A),11 chil-dren with HSPN presenting with microhematuria(Group B),11 children with HSPN presenting with micro-hematuria and proteinuria (Group C) and 11 healthy children (control group). Flow cytometry was per-formed to detect the percentages of CD19+B cells and their subsets,CD19+B cells and CD19+CD38+B cells secreting different Ig classes,CD19+CD40+B cells and their subsets and Tfh cells expressing CD40 ligand (CD40L). Results Compared with the control group,the percentages of CD19+CD86+B,CD19+CD138+B and CD40L+Tfh cells significantly increased in Group C(P<0.05) and slightly increased in Groups A and B (P>0.05). No significant difference in the percentages of CD19+B cells, CD19+CD27+B cells, CD19+B cells or CD19+CD38+B cells expressing IgG, IgM, IgD, CD19+B cells or CD19+B cell subsets secreting CD40 was found between the control group and Groups A,B and C(P>0.05). Moreover,the percentages of CD19+B and CD19+CD38+B cells secreting IgA and IgE in Groups A,B and C were higher than those in the control group(P<0.05). Secretion of IgA by CD19+B and CD19+CD38+B cells were positively correla-ted with the expression of CD40L by Tfh cells(P<0.05). Conclusion Tfh cell-mediated abnormal expres-sion of CD40/CD40L might play an important role in the development of HSP and be related to the clinical severity of renal involvement in HSPN.
