Pathogenicity of ABC transporter SSU05 0946 of Streptococcus suis serotype 2
10.3760/cma.j.issn.0254-5101.2017.12.001
- VernacularTitle:猪链球菌2型ABC转运蛋白SSU050946的致病机制研究
- Author:
Yaya PIAN
1
;
Simei REN
;
Zhenxiang GAO
;
Jingjing NIE
;
Ran ZHANG
;
Jihong HU
Author Information
1. 100730,北京医院 国家老年医学中心 卫生部临床检验中心
- Keywords:
Streptococcus suis type 2;
Whole blood killing;
Gene knockout;
Virulence factor
- From:
Chinese Journal of Microbiology and Immunology
2017;37(12):885-890
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a mutant strain of Streptococcus suis type 2 05ZYH33 express-ing ABC transporter SSU05 0946 and to study the pathogenicity of ABC transporter SSU05 0946 for better understanding the immune evasion strategies by Streptococcus suis. Methods Genome of the Streptococcus suis type 2 05ZYH33 strain was extracted and used as a template to amplify SSU05 0946 upstream and down-stream homeodomains. Chloramphenicol-resistance gene was amplified by using pSET1 plasmid as the tem-plate. These three amplified fragments were fused and integrated with the thermo-sensitive plasmid pSET4s by using overlap extension PCR. Homologous recombination method was used to construct the mutant strain 05ZYH33Δ0946. Differences between the mutant and wild type strains were evaluated through bacterial ad-hesion assay,whole blood killing assay and challenge test in mice and piglets. Results The mutant strain 05ZYH33Δ0946 was successfully constructed. Results of bacterial adhesion assay demonstrated that SSU05 0946 was not involved in the adherence of Streptococcus suis to human epithelial cells. SSU05 0946 was an ovel anti-phagocytic factor and virulence factor of Streptococcus suis. Conclusion Streptococcus suis type 2 ABC transporter SSU05 0946 is a newly discovered virulence factor of Streptococcus suis, playing an impor-tant role in the evasion of host innate immunity by Streptococcus suis.
