The effect of radiofrequency-induced hyperthermia on the expression of IL-1 beta and TNF-alpha in the blood of rabbits with knee osteoarthritis
10.3760/cma.j.issn.0254-1424.2018.07.001
- VernacularTitle:射频热疗对膝骨关节炎实验兔血液中白细胞介素-1β及肿瘤坏死因子-α表达的影响
- Author:
Zhaohua FU
1
;
Jiaojiao ZHAO
;
Qingqing CAO
;
Jiao KONG
;
Yongfei WU
;
Fei ZHANG
Author Information
1. 内蒙古医科大学第三附属医院(内蒙古包钢医院)康复医学科
- Keywords:
Knee osteoarthritis;
Radiofrequency hyperthermia;
Interleukin-1β;
Tumor necrosis factor-α
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2018;40(7):481-485
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of radiofrequency-induced hyperthermia on the morphology of articular cartilage and any changes in serum-1 interleukin-1 (IL-1β) and tumor necrosis factor-alpha (TNF-α) in the process of knee osteoarthritis in rabbits.Methods Fifty-four male rabbits were selected and knee osteoarthritis was introduced to their right hind limbs using the modified Huhh model.They were then randomly divided into a model group,a cervus and cucumis polypeptide (CCP) group and a radiofrequency thermotherapy (RT) group,each of 18.The CCP group was injected with deer melon peptide intramuscularly.The RT group was given radiofrequency hyperthermia treatment.The model group was not provided with any special treatment.On the 7th,13th and 19th day of the treatment,6 rabbits in each group were sacrificed to resect the right medial femoral condyle cartilage.The morphological characteristics of the cartilage were evaluated using the modified Mankins score,while the content of IL-1βand TNF-α in the serum were detected using enzyme-linked immuno-sorbent assays (ELISAs).Results At the same time points,the average Mankins score and the average content of IL-1βand TNF-α in the serum of the model group were significantly higher than in the CCP group,with the values in the latter group significantly higher than in the RT group.In the RT group,the average Mankins score,as well as the IL-1 beta and TNF-alpha levels in the serum,decreased significantly with longer treatment.Conclusion Radiofrequency-induced hyperthermia is superior to deer melon polypeptide in treating knee osteoarthritis,at least in rabbits.Its therapeutic mechanism may be related to the control of serum IL-1 beta and TNF-alpha levels.
