The effects of the inter-stimulus interval in magnetic stimulation on astrocyte migration and its mechanism
10.3760/cma.j.issn.0254-1424.2018.05.002
- VernacularTitle:不同间歇时间磁刺激对星形胶质细胞迁移能力的影响及机制分析
- Author:
Jun GUO
1
;
Zhe LI
;
Jiahong FAN
;
Ganghua GUO
;
Xiaoli LI
;
Chenxia GUAN
;
Lin YUE
Author Information
1. 郑州大学第五附属医院康复医学科
- Keywords:
Magnetic stimulation;
Astrocytes;
PEA-15;
Migration
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2018;40(5):325-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effect of the inter-stimulus interval (ISI) in magnetic stimulation (MS) on astrocyte migration and its related mechanism.Methods Cultured astrocytes were treated with intermittent MS with intervals of 1,5 and 10 seconds.The PEA-15 inhibitor BisI (10 μmol/ml) and the ERK1/2 inhibitor U0126 (10 μmol/ml) were administered and cell migration assays evaluated the astrocytes' migration.The expression of phosphorylated ERK1/2 and PEA-15 was detected using Western blotting.Results The 1 second interval significantly facilitated astrocyte migration,the phosphorylation of PEA-15 and ERK1/2,and the expression of MMP-9 (browse matrix metalloproteinase-9).The addition of Bis I significantly reduced the production of phosphorylated ERK1/2 and MMP-9,as well as astrocyte migration induced by MS.In addition,pretreatment with U0126 also significantly decreased the astrocyte migration induced by MS.Conclusion 1s-ISI MS can induce PEA-15 activation and subsequently lead to ERK1/2 phosphorylation and upregulation of MMP-9,which may contribute to the migration of astrocytes.
