Effects of magnetic stimulation on astrocyte migration
10.3760/cma.j.issn.0254-1424.2018.02.001
- VernacularTitle:磁刺激通过星形胶质细胞磷酸化蛋白影响星形胶质细胞迁移
- Author:
Chenxia GUAN
1
;
Ganghua GUO
;
Yumin ZHAO
;
Zhe LI
Author Information
1. 河南郑州大学第五附属医院康复医学科
- Keywords:
Magnetic stimulation;
Astrocytes;
Phosphorylated protein;
Cell transfection
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2018;40(2):81-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of the trauscranial magnetic stimulation on the migration of phosphoprotein-enriched astrocytes-15kDa (PEA-15).Methods Third or fourth generation rat astrocytes cuhured in vitro were divided into a control group,a transfected group,a magnetic stimulation group and a transfected + magnetic stimulation group.The control group was undergone transfect of negative siRNA.In transfected group and the transfected + magnetic stimulation group the liposome in the astrocyte was transfected instantly with chemically synthesized PEA-15 siRNA,so as to interfere with the expression of PEA-15 protein.Magnetic stimulation was applied to both tranfected and transfected + magnetic stimulation groups 24 h after plating of astrocytes at 1 Hz and 60％ the maximal output of the stimulator.Cell scratch tests were used to assess the astrocytes' migration,and Western blotting was applied to detect the expression of PEA-15 and protein phosphorylation.Results Compared with the control group,the expression of PEA-15 protein decreased significantly in the transfected groups.The cell migration in the transfected group,the magnetic stimulation group,and the transfected + magnetic stimulation group was significantly greater than in the control group.Compared with the control group,the phosphorylation of PEA-15 increased significantly in the magnetic stimulation group.Conclusion When PEA-15 expression is interfered with,the migration of astrocytes increases significantly.Magnetic stimulation may promote the migration of astrocytes by enhancing PEA-15 phosphorylation.
