G protein?coupled estrogen receptor reduces renal ischemia?reperfusion injury by improving diastolic function of renal interlobular artery
10.3760/cma.j.issn.1001?7097.2018.11.006
- VernacularTitle:G蛋白偶联雌激素受体通过改善肾叶间动脉舒缩活动减轻肾脏缺血再灌注损伤
- Author:
Yuechen CHANG
1
;
Ziwei HAN
;
Ying ZHOU
;
Yang ZHANG
;
Ziyi FENG
;
Ketao MA
;
Zhiqin ZHANG
;
Li LI
;
Junqiang SI
Author Information
1. 832000,新疆石河子大学医学院基础医学系
- Keywords:
Reperfusion injury;
Receptor;
estrogen;
Nitric oxide;
Renal interlobar artery
- From:
Chinese Journal of Nephrology
2018;34(11):838-844
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of G protein?coupled estrogen receptor (GPER) on the diastolic function of renal interlobular artery and reduce renal ischemia?reperfusion injury in rats. Methods Female ovariectomized rats were divided into control group; ischemia?reperfusion injury (IRI) group;GPER?specific agonist (G1) intervention group;GPER?specific blocker+GPER?specific agonist (G15+G1) intervention group. Histopathological examination (HE staining), renal function test and Paller score were used to identify the success of the model and the degree of kidney damage. In vitro microvascular pressure diameter measuring instrument was used to detect the relaxation and contraction activity of renal interlobular artery in each group. Immunofluorescence technique was used to observe the expression of GPER on the renal interlobular artery. Westernblotting was used to detect the expression of GPER protein in renal interlobular artery of rats in each group. The NO content was determined by a nitrate reductase method. Results Compared with IRI group, serum BUN, Scr level and Paller score in G1 intervention group were significantly decreased (all P<0.05). The systolic rate of renal interlobar artery was significantly increased [(40.76 ± 1.57)％ vs (29.78 ± 1.87)％, P<0.05]. The results of immunofluorescence showed that GPER was expressed in renal interlobular artery smooth muscle cells and endothelial cells, and the expression of IRI group was higher than that of the control group. The expression of G15+G1 intervention group was lower than that of G1 intervention group (all P<0.05). Compared with the IRI group, the NO content in the G1 intervention group increased significantly (all P<0.05). Conclusions During renal ischemia ?reperfusion injury, GPER may regulate the systolic and diastolic activity of the renal interlobar artery by increasing the content of NO, so as to alleviate the renal ischemia?reperfusion injury.
