Study of T cell receptor in patients with acute rejection after renal transplantation
10.3760/cma.j.issn.1001-7097.2018.04.009
- VernacularTitle:肾移植术后急性排斥反应相关的T细胞受体免疫组库变化
- Author:
Jiaxing ZHANG
1
;
Huaizhou CHEN
;
Liusheng LAI
;
Lei WANG
;
Weiguo SUI
Author Information
1. 南方医科大学附属桂林医院肾脏科
- Keywords:
Receptors,antigen,T-cell;
Complementarity determining regions;
Kidney transplantation;
Graft rejection;
Immune repertoire;
High-throughput sequencing
- From:
Chinese Journal of Nephrology
2018;34(4):281-287
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the immune status of acute rejection recipients,and to improve the short-term and long-term survival rate of renal transplant recipients and grafts,and to investigate dynamically the changes in the immune repertoire of patients with acute rejection.Methods Combined multiplex PCR amplification technique and high throughput sequencing technique,the TCR β chain complementarity determining region 3(CDR3)diversity and repertoire characteristics at different time points during renal transplantation were analyzed,in order to reveal the immunological characteristics of T lymphocytes in patients with acute rejection.Results The diversity of TCR CDR3 in acute rejection patients was reduced to the lowest one day after surgery.The diversity of TCR CDR3 before acute rejection was higher than before.The acute rejection-related upregulated TCR CDR3 amino acid sequences were screened out.In addition,TCR beta chain V and J subfamily showed the phenomenon of advantage usage in pre-acute rejection,which may be due to T cell recognition of transplanted kidney antigens in vivo.Conclusions The immune diversity of patients with acute rejection is significantly lower.In addition,TCR beta chain V and J subfamily show the phenomenon of advantage usage.
