Expression of ANLN in HCC and predictive value for long-term survival of patients after liver transplantation
10.3760/cma.j.issn.0254-1785.2018.05.001
- VernacularTitle:肌动蛋白结合蛋白在肝细胞肝癌中的表达及其对肝移植远期存活的预测价值
- Author:
Longhui ZHANG
1
;
Zhao LI
;
Dong WANG
;
Gang WANG
;
Dingbao CHEN
;
Lei HUANG
;
Jie GAO
;
Xisheng LENG
;
Jiye ZHU
Author Information
1. 100044,北京大学人民医院肝胆外科北京大学器官移植研究所北京市肝癌肝硬化基础研究重点实验室
- Keywords:
ANLN;
Hepatocellular carcinoma;
Liver transplantation;
Independent risk factor
- From:
Chinese Journal of Organ Transplantation
2018;39(5):259-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of ANLN in HCC and study the clinical value of ANLN expression for HCC patients after liver transplantation.Methods The protein and mRNA expression of ANLN was detected by immunohistochemistry and RNA-seq from TCGA respectively.Chi-square test and t test were used for correlation analysis between ANLN expression and clinicopathological characteristics.The predictive value of ANLN expression for HCC patients after liver transplantation was estimated by log-rank test and cox proportional hazards regression model.Results The positive protein expression rate of ANLN in HCC detected by immunohistochemistry was 37.0％ (34/92),significantly higher than 6.5 ％ (6/92) in para-carcinoma non-tumor tissue (P＜0.001,x2 =25.044).Upregulation of ANLN mRNA expression in HCC was also detected by the analysis of RNA-seq from the TCGA (P ＜ 0.000 1).The positive ANLN protein expression was positively correlated with AFP＞400 ng/L (P＜0.001,x2 =11.952) and tumor size ＞8 cm (P =0.034,x2 =4.506).The independent risk factors for poorer 5-year survival of patients after liver transplantation were confirmed,including positive ANLN protein expression (P =0.031,OR =1.965,95 ％CI =1.064-3.630),tumor size ＞8 em (P =0.003,OR =2.841,95 ％CI =1.437-5.617),worse differentiation degree (P =0.001,OR =3.613,95％ CI =1.646-7.928),peritumor intravascular cancer emboli (P =0.041,OR =1.896,95％CI =1.028-3.498) and tumor necrosis or hemorrhage (P=0.010,OR=2.195,95 ％CI=1.211-3.979).Conclusion The expression of ANLN in HCC is upregulated and the positive protein expression indicates the poor prognosis for long-term survival of patients after liver transplantation.
