Value of CT perfusion in different degrees of hepatic warm ischemia-reperfusion injury of rabbits
10.3760/cma.j.issn.0254-1785.2018.03.008
- VernacularTitle:CT灌注成像评价兔肝不同程度热缺血再灌注损伤的研究
- Author:
Lu XU
1
;
Zhiqiang CHU
;
Wenjuan YU
;
Qian JI
Author Information
1. 300192,天津医科大学一中心临床学院
- Keywords:
Hepatic;
Ischemia;
Reperfusion injury;
Imaging,CT;
Rabbits
- From:
Chinese Journal of Organ Transplantation
2018;39(3):164-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prospectively investigate the changes of hepatic CT perfusion parameters and the correlation with serum amylase (AST,ALT,LDH,SOD,MAD and MPO) in rabbits hepatic warm ischemia-reperfusion injury (WIRI) models undergoing different warm ischemia time.Methods New Zealand rabbits underwent 40 min and 60 min of right posterior lobe ischemia followed by 6 h reperfusion (n =10 each),named as W1,W2 groups respectively.Ten rabbits served as normal controls (W0).All the rabbits were given CT perfusion protocol.The perfusion indices of arterial flow (AF),portal flow (PF),and perfusion index (PI) were measured after obtaining perfusion index maps on the workstation.Blood samples were taken from the rabbit ear vein to measure the levels of ALT,AST,and LDH.The contents of SOD,MDA and MPO in liver tissues were determined,and histopathological changes were examined.Results Significant differences were found between the group W0,W1and W2 in the infarcted areas and non-infarcted areas by PI and AF and PF (P＜0.05),except that in the infarcted areas the AF value betwween the group W0 and W2,the PF value between the group W1 and W2 had no statistical difference,and in the non-infarcted areas the difference of the PI and AF value betwween the group W1 and W2,the PF value between the group W0 and W2 was not significant eihter.In the infracted area,PI and PF of WIRI groups positively correlated with biochemical parameters.AF was not correlated with biochemical parameters.In the non-infracted area,PI,AF was correlated with biochemical parameters,PF was not correlated with biochemical parameters.The PI and PF value could efficiently diagnose rabbit hepatic WIRI in the infarcted area (for PI,AR and PR,AUC=0.965,0.736 and 1.00 respectively),and PI and AF value could efficiently diagnose rabbit hepatic WIRI in the non-infarcted area (for PI,AF and PF,AUC=0.938,0.993 and 0.618 respectively).Conclusion CT perfusion can dynamically monitor the hemodynamic changes after WIRI and may suggest potential microcirculatory disturbances.
