Effects of mycophenolate mofetil on the intestinal gamma delta T cells and expression of serum IL-6 and TNF-alpha in mice

VernacularTitle:麦考酚酸酯对小鼠肠道γδT淋巴细胞及血浆IL-6、TNF-α表达的影响
Author: Qishun YANG ^{1
,

2} ; Jiacai YANG ; Xu WANG ; Chibing HUANG
Author Information

1. 400038重庆,第三军医大学新桥医院泌尿外二科
2. 解放军第281医院泌尿外科
Keywords: Mycophenolate mofetil; Enteritis; Gamma delta T cell; Interleukins 6; Tumor necrosis factorα; Mice
From: Chinese Journal of Organ Transplantation 2018;39(3):158-163
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of mycophenolate mofetil (MMF) on the changes of intestinal gamma delta (γδ) T cells and the secretion of plasma IL-6 and TNF-α.Methods Adult male C57BL/6 (C57) mice were randomly divided into two groups by SAS 9.1.3 software:normal saline (NS) group and MMF group.The changes of γδ T cells in each intestinal segment of mice were detected by flow cytometry.ELISA was used to determine the levels of serum IL-6 and TNF-α.Results As compared with the NS group,the ratio of γδ T cells in the epithelial cell to lymphocyte (IEL) of the intestinal tract decreased,and the concentrations of plasma IL-6 and TNF-α increased in MFF group (P＜0.05 for all).Conclusion In mycophenolic acid-related enteritis,the proportion of γδ T cells in intestinal IEL decreased,and the secretion of IL-6 and TNF-α increased.