Triptolide ameliorates transplant arteriosclerosis in rat aorta allograft by inhibiting the expression of NO synthase
10.3760/cma.j.issn.0254-1785.2017.12.008
- VernacularTitle:雷公藤内酯醇通过抑制一氧化氮合成酶2表达减缓大鼠移植物血管病的作用
- Author:
Liwen WANG
1
;
Qimin WANG
;
Zhe WU
;
Qiuyu HUANG
;
Liangwan CHEN
Author Information
1. 福建医科大学附属协和医院心血管外科
- Keywords:
Cardiac allograft vasculopathy;
Triptolide;
nitric oxide synthase 2
- From:
Chinese Journal of Organ Transplantation
2017;38(12):741-743
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the pathological role of nitric oxide synthase 2 (NOS2) in rat allograft vascular lesions and the effects of triptolide.Methods The abdominal aorta transplantation between Wistar and SD rats was used as an animal model.Three groups were set up..the same genome group (group A),the allogeneic control group (group B),and the isogene Triptolide group (group C).The grafts were removed at 7th,28th,and 56th day after surgery.The transplant artery intima thickness was measured.The irnmunohistochemical staining was applied to observe the NOS2 expression in the vascular tissue layers.The integral optical density value in each group was calculated.Results The arterial intima of transplants at 28th and 56th day postoperation was thickened,and that was thickest in group C among the three groups (P＜0.05).There was significant difference in intima thickness and integral optical density between group C with groups A and B (P＜ 0.05).The expression of NOS2 was strongest in group B,and that in group C was significantly weaker than that in group B (P ＜ 0.05).Conclusion Triptolide is capable of slowing down the progression of graft vascular disease by inhibiting the expression of NOS2.
