Effects of interleukin (IL)-10/ transforming growth factor (TGF)-β-modified macrophages on renal ischemia reperfusion injury
10.3760/cma.j.issn.0254-1785.2017.12.007
- VernacularTitle:IL-10/TGF-β诱导的巨噬细胞对肾缺血再灌注损伤的作用研究
- Author:
Yanlong ZHAO
1
;
Puxun TIAN
;
Jin ZHENG
;
Chenguang DING
;
Yi GAO
;
Wujun XUE
;
Xiaoming DING
;
Jing LIU
;
Feng HAN
;
Xinxin XIA
Author Information
1. 710061,西安交通大学第一附属医院肾脏病医院肾移植科
- Keywords:
Renal;
Ischemia reperfusion injury;
Macrophage;
Mice
- From:
Chinese Journal of Organ Transplantation
2017;38(12):734-740
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of IL-10/TGF-β-modified macrophages on renal ischemia reperfusion injury (IRI).Methods Bone marrow-derived macrophages were modified ex vivo by IL-10/TGF-β to acquire M2c (a subset of activated macrophages).M2c were transferred into treated C57BL/6 mice by a single tail-vein injection at 6 h after renal IRI.Mice were killed on the day 3 after renal IRI.Blood samples were collected to check renal function.Kidneys were harvested to determine tubular necrosis and apoptosis by H&E staining and TUNEL assay.Immunofluorescence was performed to analyze the proliferating tubular cell nuclear antigen.Meanwhile,proinflammatory cytokines and regulatory T cells in renal tissues were analyzed with real-time PCR and flow cytometry.Results In comparison with M1,M2c expressed lower levels of MHC Ⅱ (P＜0.01),CD86 (P＜ 0.01),TNFα (P＜0.01) and IL-1β (P＜0.01) and higher level of IL-10 (P＜0.01).M2c significantly attenuated renal functional decline (P＜0.01 or 0.05),structural injury (P＜0.05),apoptosis of tubular cells (P＜0.01) and inflammation factors infiltration (P＜0.01 or 0.05).What's more,the cells could promote tubular cells proliferation (P＜0.05) and regulatory T cells expression (P＜0.01).Conclusion Our results demonstrated that M2c macrophages effectively protect against renal IRI and may become a therapeutic strategy for renal IRI.
