SE-iFISH used in the detection of circulating tumor cells in patients of hepatocellular carcinoma
10.3760/cma.j.issn.1007-631X.2018.10.018
- VernacularTitle:用SE-iFISH技术检测肝细胞癌患者外周血循环中的肿瘤细胞
- Author:
Jun SHI
1
;
Guangqiang YE
;
Yuni KE
;
Chunhui YE
;
Shiwei YANG
;
Xuan TONG
;
Jing XU
;
Jiahong DONG
Author Information
1. 102218,清华大学附属北京清华长庚医院肝胆胰中心
- Keywords:
Carcinoma,hepatocellular;
Neoplastic cells,circulating;
Survival analysis
- From:
Chinese Journal of General Surgery
2018;33(10):865-868
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between patients clinical characteristics and the number and subtype of circulating tumor cells (CTCs) from peripheral blood of perioperative hepatocellular carcinoma (HCC)patients by SE-iFISH.Methods 20 HCC patients undergoing radical resection were enrolled from June 2015 to June 2016.The SE-iFISH technique was used to separate and identify circulating tumor cells.The pathology and clinical data were used to evaluate patients survival in combination with CTCs characteristics.Results A total of 347 CTCs were detected,of which 114 were triploid,64 were tetraploid,and 165 were pentaploid.The number of preoperative CTCs and the number of preoperative triploids was significantly correlated with the presence of vascular tumor emboli (Z1 =-2.080,P =0.037,Z3 =-2.321,P =0.020) and TNM staging(Z2 =-2.148,P =0.032,Z4 =-2.526,P =0.012).Postoperative patients disease-free survival in high CTCs detection group was significantly shorter than that of CTCs low expression group (x2 1 =7.486,P =0.006,x22 =12.056,P =0.001).Conclusion Detection of the number and the specific subtypes of CTCs with SE-iFISH strategy in patients with HCC help predict treatment efficacy and prognosis.
