The effect of glycated polylysine-coupled MIP-3α-FL on the immune microenvironment of mouse liver cancer
10.3760/cma.j.issn.1007-631X.2018.07.020
- VernacularTitle:糖化多聚赖氨酸偶联MIP-3α-FL对小鼠肝癌免疫微环境的调节作用
- Author:
Haichao ZHAO
1
;
Shun GUO
;
Chongren REN
;
Jiefeng HE
;
Haoliang ZHAO
Author Information
1. 山西医科大学研究生院
- Keywords:
Carcinoma,hepatocellular;
Dendritic cells;
Transfection
- From:
Chinese Journal of General Surgery
2018;33(7):596-600
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the regulation of dendritic cells by recombinant glycated polylysine-coupled MIP-3α-FL double-gene targeting expression vector in liver cancer immune microenvironment.Methods H22 hepatocarcinoma cells were transfected with recombinant plasmid of MIP-3α-FL (shMIP-3α-FL) and injected into hepatoma model mice.The survival time,tumor size were compared.Flow cytometry was used to measure the number and phenotype of tumor infiltrating DCs.Results Western blot and ELISA demonstrated that the secretion of MIP-3α and FL in H22 cells was significantly increased after transfection with MIP-3α-FL.The survival time of the mice in the experimental group was significantly prolonged,the tumor size decreased.Flow cytometry showed that the number of tumor-infiltrating DCs in the experimental group was significantly higher than that in the control group;the expression of CD80 and CD86 in the infiltrating dendritic cells (TIDCs) was significantly higher than that of the control group.Conclusions The co-action of MIP-3α and FL can significantly promote DC accumulation,maturation,and conjugate glycosylated polylysine carriers increase the precision of targeting and enhance the antigenpresentation of the DCs.
