The modulation effect of macrophage polarization by CSF-1R inhibitor administration in colon cancer
10.3760/cma.j.issn.1007-631X.2018.06.019
- VernacularTitle:集落刺激因子-1受体抑制剂对结肠癌巨噬细胞极化的调节作用
- Author:
Lianjun ZHANG
1
;
Qing ZHU
;
Jiefang ZHANG
;
Naigang WANG
Author Information
1. 菏泽市立医院重症医学科
- Keywords:
Colonic neoplasms;
Receptor,macrophages colony-stimulating factor;
Macrophages
- From:
Chinese Journal of General Surgery
2018;33(6):508-511
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the modulation effect of macrophage polarization and tumor microenvironment by colony-stimulating factor 1 receptor (CSF-1R) inhibitor administration in mice colon carcinoma tissue.Methods 24 mice were divided into normal control group,CAC group,CAC + GW2580 group.On day 64,the mice in CAC + GW2580 group were given GW2580 by daily gavage for a week.On 71 st day,the colon tissues were collected.The expression phenotype of macrophage polarization was detected by flow cytometry,the expression of macrophage-associated protein was detected by Western blot,and the expression level of various cytokine mRNAs were detected by PCR.Results The administration of CSF-1R inhibitors in CAC mice resulted in a significant reduction in the percentage of macrophages and M2 cells and a significant increase in M1 (P ＜ 0.05).The expression of M1-related proteins CD86 was up-regulated and the expression of M2-related proteins CD206 was down-regulated (P ＜0.05).Further more,the expression of M2-related IL-4,IL-10,Arg-1 were up-regulated (all P ＜ 0.05),and the expression of M1-related IL-12,iNOS were down-regulated (all P ＜ 0.05).Conclusion CSF-1R inhibitor can effectively modulate macrophage polarization from M2 to M1 in colon tumor and transform the tumor microenvironment from immunosuppression to immunostimulation,exerting tumor inhibition.
