Effect of ectopic splitting combined with portal vein ligation on rat liver regeneration
10.3760/cma.j.issn.1007-631X.2018.05.014
- VernacularTitle:异位劈离联合门静脉结扎对大鼠肝脏再生的影响
- Author:
Kongliang ZHANG
1
,
2
;
Jing HUANG
;
Shengdong WU
;
Zunqiang XIAO
;
Xi YU
;
Yuan GAO
;
Caide LU
Author Information
1. 315100浙江省,宁波大学医学院附属李惠利东部医院肝胆胰外科
2. 解放军91715部队卫生队
- Keywords:
Liver regeneration;
Portal vein;
Cytokines
- From:
Chinese Journal of General Surgery
2018;33(5):412-415
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of ectopic splitting combined with portal vein ligation on rat liver regeneration.Methods Sixty Sprague-Dawley rats were randomly divided into four groups (n =15):portal vein ligation group (PVL group),associating liver partition with PVL group (ALPP group),associating spleen partition combined with PVL group (ASPP group) and associating kidney partition combined with PVL group (AKPP group).Hepatic regeneration rate (HRR),ALT,AST and hepatocyte proliferating cell nuclear antigen (PCNA) were detected on the 2nd,4th and 7th day after operation.Results On the 4th and 7th day,in ALPP group,ASPP group and AKPP group,there was an obvious hyperplasia (F =3.750,P ＜ 0.05;F =16.398,P ＜ 0.01) in the right middle lobe of the liver and higher PCNA (F=6.860,P＜0.01;F=3.810,P＜0.05) in the liver tissue than in PVL group.And ALPP group had significantly higher ALT and AST level on the 2nd and 4th day (ALT:F =8.812,F =21.929;AST:F =36.393,F =6.468;P ＜ 0.01).Conclusions Ectopic splitting combined with PVL can significantly promote rat's liver regeneration,possibly by inflammatory response,stress response and high expression of certain cytokines.
