Detection of intraperitoneal free cancer cells in gastric cancer patients undergoing gastrectomy and its relationship with short-term prognosis
10.3760/cma.j.issn.1007-631X.2018.05.005
- VernacularTitle:胃癌患者术中腹腔游离癌细胞的检测及其与预后的关系
- Author:
Dafang LIU
1
,
2
;
Chao SHEN
;
Yushi ZHOU
;
Zhiyuan ZHENG
;
Xin LIU
;
Yingjiang YE
;
Qiwei XIE
;
Mujun YIN
;
Xiaodong YANG
;
Kewei JIANG
;
Haipeng XIAN
;
Xiaotao ZHAO
;
Bin LIANG
;
Shan WANG
Author Information
1. 100044 北京大学人民医院胃肠外科
2. 101149 首都医科大学附属北京潞河医院血管外科
- Keywords:
Stomach neoplasms;
Peritoneal lavage;
Neoplasm metastasis;
Prognosis
- From:
Chinese Journal of General Surgery
2018;33(5):376-380
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate different methods in detecting intraperitontal free cancer cells (IFCCs) in patients with gastric cancer and to clarify the relationship between positive IFCCs and short-term prognosis.Methods A total of 119 gastric cancer patients who underwent surgical treatment were enrolled.Peritoneal lavage was performed with 300-400 ml saline respectively at three points of time:immediately after abdominal cavity entry;when surgical operation was completed;when extensive intraoperative peritoneal lavage was done.The IFCCs were detected with methods of traditional centrifugal cytology,membrane cytology,ICC and RT-PCR.The survival curve of patients with gastric cancer was drawn using Kaplan-Meier method.Results The positive rate of PLC was 16.8％,20.7％ and 11.2％ respectively at 3 timepoints (P ＜ 0.05).The positive rates of ICC were 28.6％,38.8％ and 20.7％ respectively at 3 timepoints.The positive rates of RT-PCR were 39.3％,69.5％ and 50.8％ respectively at 3 time points.The positive rate of IFCCs detected through RT-PCR was higher than that of PLC and ICC (P ＜ 0.05).The short-term prognosis of patients with positive IFCCs was worse than those with negative results detected with any three method at the timg point immediately after opening the abdomen (P ＜ 0.05).At the timg point immediately after removing the tumors,the short-term prognosis of patients with positive IFCCs detected with PLC was worse (P ＜ 0.05).Conclusion The short-term prognosis was poor in patients with positive IFCCs.It is the best time to detect IFCCs before radical resection.Surgical procedures increase the risk of shedding of IFCCs.
