Evaluation of prototype IgA rapid test on diagnosis of early active syphilis
10.3760/cma.j.issn.0412-4030.2018.05.012
- VernacularTitle:梅毒IgA快速检测试剂诊断早期现症梅毒的应用性评估
- Author:
Yan HAN
1
;
Wanhui WEI
;
Yueping YIN
;
Anderson DAVID
;
Hongchun WANG
;
Garcia L MARY
;
Van HUY
;
Xiaoyu ZHU
;
Kai CHEN
;
Xiangsheng CHEN
Author Information
1. 中国医学科学院北京协和医学院皮肤病研究所中国疾病预防控制中心性病控制中心参比实验室
- Keywords:
Syphilis;
Syphilis serodiagnosis;
Point of care testing
- From:
Chinese Journal of Dermatology
2018;51(5):372-375
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application of Burnet Institute-made prototype IgA rapid test,a kind of point-of-care (POC) testing,in the diagnosis of early syphilis.Methods Totally,455 stored serum samples in the Reference Laboratory of Sexually Transmitted Disease,the Institute of Dermatology were used to evaluate the application of the prototype IgA rapid test (IgA-POC) in the diagnosis of early syphilis.According to resluts of Treponema pallidum hemagglutination assay (TPHA),rapid plasma reagin card test (RPR),and enzyme-linked immunosorbent assay for IgM antibodies (IgM-ELISA),these stored samples were divided into 3 groups:uninfected group,previously infected group and early active syphilis group.IgA-POC test was performed in the 3 groups to evaluate its diagnostic performance for active syphilis,and researchers were blind to the group information.Results The prototype IgA-POC test had a sensitivity of 92.6％ (147/163) for the early active syphilis group,a specificity of 72.22％ (104/144) for the previously infected group,and a specificity of 97.97％ (145/148) for the uninfected group.The total specificity of the prototype IgA-POC test was 85.27％,which met the minimum requirement of WHO for the POC test.The prototype IgA-POC test showed a significantly higher sensitivity for the diagnosis of early active syphilis compared with the IgM-ELISA (59.51％,Z =6.88,P ＜ 0.05),but a significantly lower specificity for the diagnosis of previous syphilis infection compared with the IgM-ELISA (98.61％,Z =6.18,P ＜ 0.05).Moreover,no significant difference in the specificity for the diagnosis of non-infection was observed between the prototype IgA-POC test and IgM-ELISA (Z =1.16,P =0.25).Conclusion The prototype IgA-POC test has better capacity for the diagnosis of early active syphilis compared with the IgM-ELISA,so it can be applied to the screening of early active syphilis.
