Simultaneous determination of six constituents in Yitai Capsules by HPLC
10.3969/j.issn.1001-1528.2018.06.018
- VernacularTitle:HPLC法同时测定颐泰胶囊中6种成分
- Author:
Man-Man LI
1
;
Hai-Tao ZHANG
;
Wei-Rong BAI
;
Zhen-Zhong WANG
;
Wei XIAO
Author Information
1. 江苏康缘药业股份有限公司
- Keywords:
Yitai Capsules;
ginsenoside Re;
ginsenoside Rg1;
ginsenoside Rb1;
specnuezhenide;
calycosin-7-O-β-D-glucoside;
oleanolic acid;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(6):1316-1319
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of ginsenoside Re,ginsenoside Rg1,ginsenoide Rb1,specnuezhenide,calycosin-7-O-β-D-glucoside and oleanolic acid in Yitai Cap sules (Ginseng Radix et Rhizoma,Astragali Radix,Ligustri lucidi Fructus,etc.).METHODS The analysis of 70％ ethanol extract of this drug was performed on a 25 ℃ thermostatic Luna C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of methanol-O.2％ phosphoric acid flowing at 1.0 mL/minin a gradient elution manner,and the detection wavelength was set at 203 nm.RESULTS Six constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 95.58％-102.12％ with the RSDs of 0.82％-1.73％.CONCLUSION This simple and stable method can be used for the rapid quality control of Yitai Capsules.
