Simultaneous determination of five constituents in Roudoukou-8 Powder by QAMS
10.3969/j.issn.1001-1528.2018.06.016
- VernacularTitle:一测多评法同时测定肉豆蔻-8散中5种成分
- Author:
Wen-Ting JIA
1
;
Yuan-Yan ZANG
;
Na GUO
;
Yu-Hua WANG
Author Information
1. 内蒙古医科大学药学院
- Keywords:
Roudoukou-8 Powder;
ellagic acid;
eugenol;
costunolide;
dehydroroma lactone;
dehydrodiisoeugenol;
quantitative analysis of multi-components by single-marker (QAMS)
- From:
Chinese Traditional Patent Medicine
2018;40(6):1307-1312
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous content determination of five constituents in Roudoukou-8 Powder (Myristicae Semen,Auck landiae Radix,Lignum aquilariae Resinatum,etc.).METHODS The analysis of 75％ methanol extract of this drug was performed on a 30 ℃ thermostatic Apollo C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of methanol-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 225,254,273,281 nm.With eugenol as an internal standard,the relative correction factors of the other four constituents were calculated,after which the content determination was made.RESULTS Ellagic acid,eugenol,costunolide,dehydroroma lactone,dehydrodiisoeugenol showed good linear relationships within the ranges of 0.227 0-1.135 2,5.272 2-26.361 0,0.540 8-2.704 0,0.530 4-2.652 0,0.059 0-0.299 5 μg (r ＞0.999 0),whose average recoveries (RSDs) were 96.37％ (2.07％),102.19％ (2.78％),101.66％ (1.66％),103.46％ (1.17％),98.25％ (1.98％),respectively.The results obtained by QAMS approximated those obtained by external standard method.CONCLUSION This stable and reliable method can be used for the quality control of Roudoukou-8 Powder.
