Oxidant stress and opoptotic effects of anisodine hydromide on rats with chronic cerebral ischemic injury
10.3969/j.issn.1001-1528.2018.06.003
- VernacularTitle:氢溴酸樟柳碱对慢性脑缺血损伤大鼠氧化应激及细胞凋亡的影响
- Author:
Dan-Dan CHEN
1
;
Xiao-Fang XIE
;
Meng-Ting LI
;
Shi-Yang ZHANG
;
Si YU
;
Feng WAN
;
Cheng PENG
Author Information
1. 成都中医药大学药学院
- Keywords:
anisodine hydromide (AH);
chronic cerebral ischemic injury;
oxidation stress;
opoptosis;
PC12 cells
- From:
Chinese Traditional Patent Medicine
2018;40(6):1242-1248
- CountryChina
- Language:Chinese
-
Abstract:
AIM To observe the oxidant stress and opoptotic effects of anisodine hydromide (AH) on chronic cerebral hypoperfusion (CCH) rats.METHODS In vivo CCH models were established in adult male SpragueDawley rats by permanent ligation of bilateral common carotid arteries [two-vessel occlusion (2-VO)] surgery.Rats were randomly divided into six groups,sham group,model group,positive group of n-butylphthalide and sodium chloride injection,and AH groups (1.2 mg/kg high-dose group,0.6 mg/kg medium-dose group,and 0.3 mg/kg low-dose group).Antioxidant indices including the activity of SOD,CAT,LDH and iNOS and the content of GSH and NO were measured.In the in vitro trial,PC12 cells were divided into control group,model group,positive group of n-butylphthalide,and AH groups (100 μmol/L high-dose group,50 μmol/L mediumdose group,and 25 μmol/L low-dose group),and the hypoxic models were established by treating PC12 cells with CoCl2.The cells had their release of NO and LDH detected,their cellular apoptosis determined by Hochest 33342 fluorescence staining,and the expression of P53 protein identified by IF (immunofluorescence) and Western blotting method.RESULTS The in vivo trial revealed AH's enhancement in serum SOD activity and inhibition in serum iNOS activityof the CCH rats,and its power in the cerebral GSH and LDH release reduction.The in vitro trial showed the resultant lower LDH and NO release,decreased number of neuro-apoptosis,and inhibited P53 pro tein expression after AH intervention.CONCLUSION The antioxidant and antiapoptotic effects of AH on CCH rats may be associated with down regulation of P53 protein.
