Simultaneous determination of seven constituents in Gnaphalium affine
10.3969/j.issn.1001-1528.2018.05.023
- VernacularTitle:HPLC法同时测定鼠曲草中7种成分
- Author:
Jun-Bin GAO
1
;
Xuan WANG
;
Yan-Hong CHEN
;
Jun-Li LI
;
Shan-Shan L(U)
;
Lian-Na SUN
Author Information
1. 上海上药华宇药业有限公司
- Keywords:
Gnaphalium affine D.Don;
chemical constitutions;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(5):1116-1119
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of seven constituents in Gnaphalium affine D.Don.METHODS The analysis of 80％ methanol of G.affine was performed on a 30 ℃ Atlantis (C) T3 column (4.6 mm× 250 mm,5 μm),with the mobile phase comprising of acetonitrile-formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 288 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (R2 ≥0.999 8),whose average recoveries were 98.58％-103.8％ with the RSDs of 0.88％-1.74％.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of G.affine.
