Determination of six constituents in Shiyifang Vinum by HPLC
10.3969/j.issn.1001-1528.2018.05.016
- VernacularTitle:HPLC法测定十一方药酒中6种成分
- Author:
Xian-Yi SHI
1
;
Zhi-Ping WANG
;
Fang LI
;
Xue-Mei CAI
;
Xiao-Yi CHEN
;
Jie-Lian HUANG
Author Information
1. 广西中医药大学
- Keywords:
Shiyifang Vinum;
notoginsenoside R1;
ginsenoside Rg1;
ginsenoside Rb1;
asperosaponin Ⅵ;
brucine;
strychnine;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(5):1088-1093
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the content determination of six constituents in Shiyifang Vinum (Notoginseng Radix et Rhizoma,Dipsaci Radix,Carthami Flos,etc.).METHODS The content determination of notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 and asperosaponin Ⅵ was performed on a 30℃ thermostatic Inertsil(R) ODS-3 C18column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient manner,and the detection wavelength was set at 203 nm.The content determination of brucine and strychnine was conducted on a 30 ℃ thermostatic Geminni(R) C18 110(A) column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-mixed solution of 0.01 mol/L sodium heptanesulfonate and 0.02 mol/L potassium dihydrogen phosphate flowing at 1.0 mL/min in an isocratic elution manner,and the detection wavelength was set at 260 nm.RESULTS Six constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 98.52％-99.96％ with the RSDs of 2.0％-2.3％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Shiyifang Vinum.
