Establishment of UPLC fingerprints of Gegen Qinlian Pills and determination of seven constituents
10.3969/j.issn.1001-1528.2018.05.015
- VernacularTitle:葛根芩连丸UPLC指纹图谱建立及7种成分测定
- Author:
Yi-Han WU
1
;
Di WANG
;
Xiao-Qin YANG
;
Wei LI
;
Chao-Mei FU
;
Jin-Ming ZHANG
;
Liang ZOU
Author Information
1. 成都中医药大学药学院,四川成都611137
- Keywords:
Gegen Qinlian Pills;
fingerprints;
chemical constituents;
UPLC
- From:
Chinese Traditional Patent Medicine
2018;40(5):1083-1087
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the UPLC fingerprints of Gegen Qinlian Pills (Puerariae lobatae Radix,Scutellariae Radix,Coptidis Rhizoma and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle) and to determine the contents of puerarin,baicalein,palmatine,wogonin,daidzin,daidzein and jateorhizine.METHODS The analysis of 70％ methanol extract of this drug was performed on a 30 ℃ thermostatic Waters ACQUITY UPLC(C) BEH C18column,with the mobile phase comprising of methanol-0.1％ glacial acetic acid flowing at 0.3 mL/min in a gradient manner,and the detection wavelength was set at 260 nm.RESULTS There were twenty-five common peaks in the fingerprints of ten batches of samples with the similarities of more than 0.98.Seven constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 98.99％-103.6％ with the RSDs of 1.13％-2.03％.CONCLUSION This simple,reliable and reproducible method can be used for the quality control of Gegen Qinlian Pills.
