Miaoling Natto Capsules in protection against myocardial ischemia-reperfusion injury in rats
10.3969/j.issn.1001-1528.2018.05.004
- VernacularTitle:苗岭纳豆素胶囊对大鼠心肌缺血再灌注损伤的保护作用
- Author:
Hong-Xia XIANG
1
;
Peng-Jiao WANG
;
Yan LIU
;
Shuo ZHANG
;
Xiao-Xia MENG
;
Jia WANG
;
Xiu-Li GAO
Author Information
1. 贵州医科大学药学院
- Keywords:
Miaoling Natto Capsules (MNC);
fibrinolytic kinase DCK;
ischemia-reperfusion;
myocardial infarction range
- From:
Chinese Traditional Patent Medicine
2018;40(5):1025-1030
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the protective effects of Miaoling Natto Capsules (MNC) on myocardial ischemia-reperfusion injury (MIR) in rats.METHODS Forty-eight healthy male Sprague-Dawley (SD) rats randomly divided into sham group,model group,positive control group (propranolol),MNC groups (low dose,medium dose,and high dose groups) underwent corresponding 7-day oral administration at a frequency of twice daily (rats of the sham group and the model group were dosed with saline water at 1 mL/100 g).Anesthetized by 8％ chloral hydrate,rat models were made by left anterior descending coronary artery ligation,30 min coronary occlusion followed by 3 h of reperfusion for ST segments and T waves monitoring,and rats in the sham group were performed opening and suture procedures.The rats had their serum levels of acetic transaminase (AST),lactate dehydrogenase (LDH),creatine kinase isoenzyme (CK-MB),cardiac troponin-Ⅰ (cTn-Ⅰ),superoxide dismutase (SOD),malondialdehyde (MDA),and tumor necrosis factor-α (TNF-α) detected,real time ECG changes monitored and myocardial infarction area assessed by TTC.RESULTS Compared with the sham group,the model group was observed with markedly elevated ST segments or high T waves rise,significantly increased activities of CK-MB,LDH,AST and the content of cTnⅠ,MDA,TNF-α,and decreased activity of SOD (P ＜ 0.01 or P ＜ 0.001).Compared with the model group,the positive control group and the low,medium and high dose MNC groups achieved controlled ST segments elevation or greater T waves amplitude,significantly decreased activities of CKMB,LDH,AST and the content of cTnⅠ,MDA,TNF-α increased activity of SOD (P ＜0.01 or P ＜0.05) and mycocardial infact range reduction (P ＜ 0.001).CONCLUSION MNC is protective to rats with myocardial ischemia-reperfusion injury.
