An investigation of oxidative stress based effect and mechanism of Shenfu Injection on atherosclerosis of ApoE-/-mice
10.3969/j.issn.1001-1528.2018.05.002
- VernacularTitle:基于氧化应激探讨参附注射液延缓ApoE-/-小鼠动脉粥样硬化的作用及机制
- Author:
Ying-Xin SUN
1
;
Tong ZHANG
;
Dan-Bin WU
;
Chun SUN
;
Bin LU
;
Xi-Juan JIANG
Author Information
1. 天津中医药大学病理教研室
- Keywords:
atherosclerosis (AS);
ApoE knockout mice;
oxidative stress (OS);
traditional Chinese medicine;
Shenfu Injection
- From:
Chinese Traditional Patent Medicine
2018;40(5):1015-1019
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effect of Shenfu Injection on atherosclerosis (AS) models of high-fat apolipoprotein E-deficient (ApoE-/-) mice,and to explore its anti-atherosclerosis mechanism through the observation of oxidative stress (OS) variation.METHODS C57 mice were used as controls.ApoE-/-mice fed with 20-week high fat diet were randomly divided into model group,Shenfu group for subsequent 4-week continuous corresponding intervention,after which the mice had their blood lipid levels measured,their levels of MPO and NOX4 identified by ELISA,and their T-SOD activity determined by hydroxylamine method,their MDA level detected by TBA,their plaque formation observation achieved by HE staining of aortic gross and red O of all the aorta,and their Nrf2 mRNA expression detected by real time qPCR method.RESULTS Compared with the control group,the model group manifested with increased contents of TG,TC,LDL,decreased HLD;decreased activity of SOD,increased contents of MPO,NOX4 and MDA,and down-regulated expression of aortic Nrf2 and Keap1 mRNA.Compared with the model group,Shenfu Injection group was observed with no obvious blood lipid level change,but a reduction of plaque area,and an effective inhibition on OS as revealed by improved levels of Nrf2 and Keap1 mRNA.CONCLUSION Shenfu Injection can activate Nrf2 and interfer the relevant enzymes,thus prevents the atherosclerosis progression through OS reduction.
