Determination of six constituents in Qingkailing Freeze-Dried Powder for Injection by HPLC
10.3969/j.issn.1001-1528.2018.04.016
- VernacularTitle:HPLC法测定注射用清开灵(冻干)中6种成分
- Author:
Fang WANG
1
;
Peng DU
;
Hong-Mei HUANG
;
Xiang-Jun MAO
;
Qian-Li XU
Author Information
1. 遵义医学院
- Keywords:
Qingkailing Freeze-Dried Powder for Injection;
adenosine;
chlorogenic acid;
gardenoside;
baicalin;
hyodeoxycholic acid;
cholic acid;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(4):848-852
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the content determination of six constituents in Qingkailing Freeze-Dried Powder for Injection (cholic acid,hyodeoxycholic acid,Bubali Cornu,etc.).METHODS The content determination of adenosine,chlorogenic acid and gardenoside was performed on a 30 ℃ thermostatic XBridge C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-water (containing 0.1％ formic acid) flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 254 nm.The content determination of baicalin,hyodeoxycholic acid and cholic acid was performed on a 35 ℃ thermostatic XBridge C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of methanol-water (containing 0.1％ formic acid) flowing at 1.0 mL/min in a gradient elution manner.RESULTS Six constituents showed good linear relationships within the ranges of 2.244-56.108,2.658-66.445,4.347-108.682,122.01-1 016.75,131.94-1 099.50,152.22-1 268.50 μg/mL (r ＞ 0.999 0),whose average recoveries (RSDs) were 101.1％ (0.46％),98.0％ (1.74％),99.7％ (0.15％),100.9％ (1.31％),98.1％(0.18％),98.2％ (1.61％),respectively.CONCLUSION This stable and reproducible method can be used for the quality control of Qingkailing Freeze-Dried Powder for Injection.
