Optimization of combined enzymatic extraction for alkaloids and polysaccharides from Dendrobium nobile
10.3969/j.issn.1001-1528.2018.04.012
- VernacularTitle:金钗石斛中生物碱与多糖联合酶提工艺的优化
- Author:
Jiao AO
1
;
Jia-Ke BAO
;
Yu-Ji XIA
Author Information
1. 贵阳中医学院
- Keywords:
Dendrobium nobile Lindl.;
alkaloids;
polysaccharides;
combined enzymatic extraction;
orthogonal test
- From:
Chinese Traditional Patent Medicine
2018;40(4):830-834
- CountryChina
- Language:Chinese
-
Abstract:
AIM To optimize the combined enzymatic extraction for alkaloids and polysaccharides from Dendrobium nobile Lindl..METHODS With enzyme consumption,enzymolysis temperature,enzymolysis time and solidliquid ratio as influencing factors,contents of dendrobine,total alkaloids and polysaccharides as evaluation indices,orthogonal test was applied to optimizing the combined enzymatic extraction.RESULTS The optimal conditions for papain extraction were determined to be 0.10 g for enzyme consumption,45 ℃ for enzymolysis temperature,2 h for enzymolysis time,and 1 ∶ 50 for solid-liquid ratio,the contents of dendrobine,total alkaloids and polysaccharides were 3.495 5,4.341 8 and 35.898 7 mg/g,respectively.The optimal conditions for cellulase extraction were determined to be 0.30 g for enzyme consumption,50 ℃ for enzymolysis temperature,2 h for enzymolysis time,and 1 ∶40 for solid-liquid ratio,the contents of three constituents were 3.514 8,4.351 3 and 36.331 2 mg/g,respectively.The optimal conditions for pectinase extraction were determined to be 0.45 g for enzyme consumption,55 ℃ for enzymolysis temperature,2.5 h for enzymolysis time,and 1 ∶ 40 for solid-liquid ratio,the contents of three constituents were 3.524 4,4.452 8 and 26.324 2 mg/g,respectively.CONCLUSION This stable and reliable method can be used for the rapid combined enzymatic extraction for alkaloids and polysaccharides from D.nobile.
