The active substance of Rostellularia procunbens and its mechanism in inhibiting nephritis cell proliferation
10.3969/j.issn.1001-1528.2018.04.004
- VernacularTitle:爵床抑制肾炎细胞增殖的物质基础及作用机理
- Author:
Ying-Jie FU
1
;
Yue LI
;
Jiao-E GAN
;
Zong-Chao HONG
;
Yan-Fang YANG
;
He-Zhen WU
Author Information
1. 湖北中医药大学药学院
- Keywords:
Rostellularia procumbens (L.) Nees;
nephritis cells;
justicidin A;
TLR4/NF-κB signalling pathway;
cytokines
- From:
Chinese Traditional Patent Medicine
2018;40(4):783-787
- CountryChina
- Language:Chinese
-
Abstract:
AIM To identify the active anti-chronic nephrotic substance of Rostellularia procunbens (L.) Nees,and to study its mechanism.METHODS Rat glomerular mesangial cells (HBZY-1) were developed into nephrotic cell models by LPS.The activities of extract of petroleum ether,ethyl acetate,n-butanol and water were screened by MTT and ELISA kit,after which isolation and purification of the various compounds were achieved,and their effects on the expression of TLR4/NF-κB pathway were determined by Western blot.RESULTS Both extracts of petroleum ether and ethyl acetate exhibited anti-nephrotic activity,and Justicidin A was determined to be the active compound inhibiting both the proliferation of mesangial cells and the release of cytokines to some extent.CONCLUSION Rostellularia procunbens (L.) Nees may inhibit the expression of inflammatory proteins through TLR4/NF-κB signalling pathway to prevent chronic nephritis.
