Analysis and comparison of HPLC fingerprints of wanai and Artemisiae argyi from thirty-one growing areas by multistatistical
10.3969/j.issn.1001-1528.2018.03.030
- VernacularTitle:多元统计分析比较宛艾及31个产地艾叶HPLC指纹图谱
- Author:
Yu-Qing WANG
1
;
Guo ZHUANG
;
Xu-Xue ZHANG
;
Rong-Hui GENG
Author Information
1. 南阳医学高等专科学校科研实验中心
- Keywords:
Artemisiae argyi Levl.et Vant;
wanai;
multistatistical;
HPLC fingerprints;
similarity analysis;
cluster analysis;
principal component analysis
- From:
Chinese Traditional Patent Medicine
2018;40(3):653-658
- CountryChina
- Language:Chinese
-
Abstract:
AIM To analyze and compare HPLC fingerprints of wanai and Artemisiae argyi Levi.et Vant from thirty-one growing areas by multistatistical.METHODS The analysis of 80％ methanol extract of A.argyi was developed on a 30 ℃ thermostatic Agilent TC-C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-water (containing 0.2％ methanoic acid) flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 330 nm.RESULTS There were eighteen,twenty-five common peaks in the fingerprints of thirty-one batches of A.argyi,fifteen batches of wanai,respectively,with the similarities all more than 0.900.The similarities of thirty-one batches of samples from different growing area were good and together as a category except samples from Dengzhou city,Luohe city and Anhui province.Fifteen batches of wanai samples got together with Qiai among them.The cumulative contribution rate of the four principal components from A.argyi was 86.049％.Twelve batches of wanai samples had higher scores than Qiai.CONCLUSION This stable and reliable method can be used for the quality control of A.argyi.
