Simultaneous determination of huperzine A and huperzine B in Seventeen species from Huperzioideae subfamily by HPLC
10.3969/j.issn.1001-1528.2018.03.027
- VernacularTitle:HPLC法同时测定17种石杉亚科植物石杉碱甲、乙
- Author:
Hui ZHAO
1
;
Lan-Fang QIN
;
Yun-Feng HUANG
Author Information
1. 广西中医药大学
- Keywords:
Huperzioideae subfamily;
huperzine A;
huperzine B;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(3):637-642
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of huperzine A and huperzine B from seventeen species Huperzioideae subfamily (including 2 ineditus new species).METHODS The analysises of tartaricacid extracts of huperzine A and huperzine B were performed on a room temperature column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of methanol-spiritus mindereri flowing at 1.0 mL/min in a isocratic elution manner,and the detection wavelength was set at 310 nm.The contents were determined by external standards.RESULTS Huperzine A and huperzine B showed good linear relationships within 0.002 0-0.30 μg,0.001 8-0.27 μg (r=0.999 9),whose average recoveries were 103.86％,101.3％ with the RSDs of 1.85％,1.30％,respectively.Huperzine A and huperzine B were found in seventeen species,and there were significant differences in contents from species to species.The content of huperzine A in Phlegmariurus hamiltonii was the highest,which reached up to 0.22％,with higher levels of it in Phlegmariurus cryptomerianus,Phlegmariurus petiolatus,and Phlegmariurus phlegmaria;The content of huperzine B was the highest in P.phlegmaria.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of huperzine A and B from Huperzioideae subfamily.
