Establishment of UPLC fingerprints of Jasminum elongatum and determination of two constituents
10.3969/j.issn.1001-1528.2018.03.026
- VernacularTitle:扭肚藤UPLC指纹图谱建立及2种成分测定
- Author:
Ai-Li XU
1
;
Xiao-Li BI
;
Bu-Ming LIU
;
Jie-Yi JIANG
;
Zhao CHEN
;
Ping DING
Author Information
1. 广州中医药大学
- Keywords:
Jasminum elongatum (Bergium) Wild.;
UPLC fingerprints;
isochlorogenic acid B;
methylcaffeate
- From:
Chinese Traditional Patent Medicine
2018;40(3):632-637
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the UPLC fingerprints of Jasminum elongatum (Bergium) Wild.and to determine the contents of isochlorogenic acid B and ethylcaffeate.METHODS The analysis of methanol extract of J.elongatum was developed on a 25 ℃ thermostatic Agilent Ecplise XDB-C18 column (2.1 mm × 100 mm,1.7 μm),with the mobile phase comprising of acetonitrile-0.1％ methanoic acid flowing at 0.5 mL/min in a gradient elution manner,and the detection wavelength was set at 260 nm.RESULTS There were eighteen common peaks in the fingerprints of ten batches of samples,with the similarities of more than 0.85.Isochlorogenic acid B and ethylcaffeate showed good linear relationships within the ranges of 7.67-38.35 μg/mL (R2 =0.999 4),9.60-96.0 μg/mL (R2 =0.999 7),whose average recoveries were 98.61％,99.09％ with the RSDs of 0.84％,1.25％,respectively.CONCLUSION This stable and reliable method can be used for the quality control of J.elongatum.
