Effect and mechanism of methanolic extract of Eupatorium to model rats with chronic muscle injury
10.3969/j.issn.1001-1528.2018.03.006
- VernacularTitle:泽兰甲醇提取物对慢性软组织损伤模型大鼠的影响及其机制
- Author:
Jian-Lian GAO
1
;
Han-Wei LI
;
Zhi-Jian DENG
;
Yi-Zhong LU
;
Xin ZHANG
;
Jin-Cai WANG
;
Kui JIA
Author Information
1. 新乡医学院第一附属医院
- Keywords:
Eupatorium;
chronic soft tissue injury;
inflammatory cytokines;
oxidative stress;
Collagen-Ⅰ/Ⅲ;
VEGF;
bFGF
- From:
Chinese Traditional Patent Medicine
2018;40(3):537-543
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effect and mechanism of methanolic extract of Eupatorium (MEOE) to model rats with chronic soft tissue injury.METHODS The model rats were established by mechanical injury and a subsequent two-week normal feeding for respective administration of high,medium and small dosage of MEOE once a day successively for 14 days.An array of indices,the level of superoxide dismutase (SOD),malondialdehyde (MDA),prostaglandin E2 (PGE2),nitric oxide (NO),interleukin-6 (IL-6) and histamine,the expression of tumor necrosis factor-alpha (TNF-α),nitric oxide (NO) and Collagen-Ⅰ/Ⅲ,and the activity of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were measured to analyze the effect of MEOE to model rats with chronic muscle injury.RESULTS MEOE resulted in apparent reduction of contents of MDA,PGE2 and NO,and the levels of TNF-α and IL-6 in muscular tissue (P ＜ 0.05),significantly increased of the SOD in muscular tissue (P ＜ 0.01),a remarkably inhibited expression of the tissue Collagen-Ⅰ/Ⅲ protein (P ＜ 0.01),and significantly improved activity of tissue VEGF and bFGF (P ＜ 0.01).CONCLUSION The certain therapeutic effects of MEOE to rats with chronic muscle injury may correlate with its influence to the levels of inflammatory factors inhibition,the oxidative stress relief,the overexpression of collagen-Ⅰ/Ⅲ inhibition,the VEGF and bFGF activity improvement,and the time spare from the repairing.
