Determination of effective components and analysis of antioxidant enzyme activities of Ampelopsis grossedentata under different breeding modes
10.3969/j.issn.1001-1528.2018.02.030
- VernacularTitle:藤茶不同繁殖方式有效成分测定及抗氧化酶活性分析
- Author:
Yao-Bing CHEN
1
;
Nian JIANG
;
Zi-Wei SUN
;
Shu-Rui TENG
;
Xiao-Jiang ZHENG
Author Information
1. 湖北民族学院林学园艺学院
- Keywords:
Ampelopsis grossedentata (Hand.-Mazz.) W.T.Wang;
breeding modes;
effective components;
antioxidant enzymes activities
- From:
Chinese Traditional Patent Medicine
2018;40(2):399-403
- CountryChina
- Language:Chinese
-
Abstract:
AIM To determine effective components of Ampelopsis grossedentata (Hand.-Mazz.) W.T.Wang under different breeding modes and to analyze the antioxidant enzyme activities.METHODS Single factor and orthogonal tests were adopted in screening the optimal extraction process of antioxidant enzymes and analyzing antioxidant enzyme activities of A.grossedentata under common cutting,two-phase cutting and tissue culture.Solvent extraction method was adopted in extracting the effective components of three breeding modes in stem leaves of A.grossedentata,whose contents were determined.RESULTS In three breeding modes,the content of polysaccharides in tissue culture seedling was the highest,while the contents of flavonoids and polyphenols in the two stage cutting seedlings were the highest.The optimal extraction process of antioxidant enzymes was determined to be 6.0 for pH,20 ℃ for processing temperature and 20 min for processing time.Antioxidant enzyme activities were in sequence of two stage cutting seedlings ＞ common cutting seedling ＞ tissue culture seedling.CONCLUSION Considering the contents of effective components and antioxidant enzyme activities,two stage cutting method is the best among 3 breeding modes.
