Simultaneous determination of four constituents in Liujing Toutong Tablets by HPLC
10.3969/j.issn.1001-1528.2018.02.021
- VernacularTitle:HPLC法同时测定六经头痛片中4种成分
- Author:
Hong-Ling DONG
1
;
Qin-Qing LI
;
Jin-Miao CHAI
;
Wen-Bin HE
;
Xiu-Ying LI
Author Information
1. 山西中医药大学
- Keywords:
Liujing Toutong Tablets;
puerarin;
ferulic acid;
imperatorin;
isoimperatorin;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(2):355-358
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Liujing Toutong Tablets (Angelicae dahuricae Radix,Magnoliae Flos,Ligustici Rhizoma et Radix,etc.).METHODS The analysis of 30％ ethanol extract of this drug was performed on a 35 ℃ thermostatic Waters C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of methanol-4％ acetic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 320 nm.RESULTS Puerarin,ferulic acid,imperatorin and isoimperatorin showed good linear relationships within the ranges of 60.6-303 μg/mL (r=0.999 9),1.59-7.95 μg/mL (r =0.999 9),1.57-7.85 μg/mL (r =0.999 9) and 0.752 5-3.762 5 μg/mL (r =0.999 7),whose average recoveries (RSDs) were 97.75％ (1.7％),97.68％ (2.3％),97.94％ (1.0％) and 98.29％ (1.6％),respectively.CONCLUSION This stable and reliable method can be used for the quality control of Liujing Toutong Tablets.
