Simultaneous determination of six constituents in Xuanmai Ganju Granules by HPLC
10.3969/j.issn.1001-1528.2018.02.020
- VernacularTitle:HPLC法同时测定玄麦甘桔颗粒中6种成分
- Author:
Ping XUE
1
;
Xin YAO
;
Li LI
Author Information
1. 常州市食品药品监督检验中心
- Keywords:
Xuanmai Ganju Granules;
harpagide;
liquiritin apioside;
liquiritin;
harpagoside;
cinnamic acid;
glycyrrhizic acid;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(2):351-354
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of six constituents in Xuanmai Ganju Granules (Scrophulariae Radix,Ophiopogonis Radix,Glycyrrhizae Radix et Rhizoma,Platycodonis Radix).METHODS The analysis of 80％ methanol extract of this drug was performed on a 35 ℃ thermostatic ZORBAX SB-C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 210,250,278 nm.RESULTS Harpagide,liquiritin apioside,liquiritin,harpagoside,cinnamic acid and glycyrrhizic acid showed good linear relationships within the ranges of 2.177-43.539 μg/mL(r =0.999 6),1.713-34.261 μg/mL (r =0.999 5),1.946-38.916 μg/mL(r =0.999 6),2.070-41.395 μg/mL(r =0.999 7),2.06-41.2 pg/mL (r =0.999 6) and 3.623-72.454 μg/mL (r =0.999 6),whose average recoveries (RS-Ds) were96.08％ (2.1％),95.55％ (2.5％),95.04％ (2.6％),94.86％ (2.7％),95.70％ (1.9％) and 95.47％ (1.9％),respectively.CONCLUSION This simple and accurate method can be used for the quality control of Xuanmai Ganju Granules.
