Simultaneous determination of seven constituents in Waicha Bailing Tincture by HPLC
10.3969/j.issn.1001-1528.2018.02.019
- VernacularTitle:HPLC法同时测定外搽白灵酊中7种成分
- Author:
Hong-Kai CHEN
1
;
Han-Zhen PEI
;
Hai-Long ZHAO
Author Information
1. 青海大学附属医院药剂科
- Keywords:
Waicha Bailing Tincture;
chemical constituents;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(2):347-350
- CountryChina
- Language:Chinese
-
Abstract:
AIM To eslablish an HPLC method for the simultaneous content determination of brazilin,(±) protosappanin B,hydroxysafflor yellow A,safflor yellow A,oxypeucedanin,imperatorin and isoimperatorin in Waicha Bailing Tincture (Angelicae dahuricae Radix,Sappan Lignum,Carthami Flos,etc.).METHODS The analysis of methanol extract of this drug was performed on a Agilent TC-C18 column (200 mm ×4.6 mm,5 μm),with the mobile phase comprising of methanol-0.1％ glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 285,403,310 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥0.999 5),whose average recoveries were 96.91％-99.59％ with the RSDs of 0.81％-1.19％.CONCLUSION This stable and reliable method can be used for the quality control of Waicha Bailing Tincture.
