Simultaneous determination of seven constituents in Supplemented Ermiao Granules by HPLC and establishment of fingerprints
10.3969/j.issn.1001-1528.2018.02.018
- VernacularTitle:HPLC法同时测定加味二妙颗粒中7种成分及指纹图谱建立
- Author:
Jing ZOU
1
;
Hui CHEN
;
Yi PENG
;
Qing-Ling REN
;
Zhi-Hui LIU
Author Information
1. 南京中医药大学第一附属医院
- Keywords:
Supplemented Ermiao Granules;
chemical constituents;
fingerprints;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(2):341-346
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of phellodendrine chloride,berberine hydrochloride,jatrorrhizine hydrochloride,(R,S)-goitrine,astilbin,hesperidin and atractylenolide Ⅲ in Supplemented Ermiao Granules (Phellodendri chinensis Cortex,Atractylodis Rhizoma,Smilacis glabrae Rhizoma,etc.) and to establish fingerprints.METHODS The analysis of 70％ methanol extract of this drug was performed on a 35 ℃ thermostatic Amethyst C18-H column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of 0.05 mol/L potassium dihydrogen phosphate-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 237,291 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r =1),whose average recoveries were 95.77％-110.36％ with the RS-Ds of 0.06％-2.94％.There were twenty common peaks in the fingerprints of fourteen batches of samples with the similarities of more than 0.990,five of which (5-hydroxymethylfurfural,phellodendrine,astilbin,hesperidin and berberine hydrochloride) were identified.CONCLUSION This stable,reliable and reproducible method can be used for the quality control of Supplemented Ermiao Granules.
