Simultaneous determination of five constituents in Baishao Formula Granules by HPLC
10.3969/j.issn.1001-1528.2018.01.023
- VernacularTitle:HPLC法同时测定白芍配方颗粒中5种成分
- Author:
Jun QIAN
1
;
Fan XIE
;
Yan-Hong SHI
;
Wen-Yan LI
;
Rui WANG
Author Information
1. 上海中医药大学中药学院
- Keywords:
Baishao Formula Granules;
paeoniflorin;
albiflorin;
paeoniflorin sulfonate;
gallic acid;
benzoic acid;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(1):117-120
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of five constituents in Baishao Formula Granules (Paeoniae Radix Alba).METHODS The analysis of aqueous extract of this drug was performed on a 30 ℃ thermostatic Phenomenex C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.05％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.RESULTS Paeoniflorin,albiflorin,paeoniflorin sulfonate,gallic acid and benzoic acid showed good linear relationships within the ranges of 0.020-0.639 mg/mL (r =0.999 8),0.005-0.172 mg/mL (r =0.999 9),0.020-0.652 mg/mL (r =1.000 0),0.003-0.097 mg/mL (r =0.999 8),0.002-0.058 mg/mL (r =0.999 7),whose average recoveries were 99.1％,98.3％,98.6％,98.1％ and 99.5％ with the RSDs of 1.86％,1.37％,1.69％,1.46％ and 2.26％,respectively.The contents of various constituents in twenty-seven batches of samples demonstrated obvious differences.CONCLUSION We should pay attention to Paeoniae Radix Alba in Baishao Formula Granules due to its unstable quality.
