Simultaneous determination of eleven constituents in Huxin Oral Liquid by LC-MS/MS
10.3969/j.issn.1001-1528.2018.01.020
- VernacularTitle:LC-MS/MS法同时测定护心口服液中11种成分
- Author:
Kun LIANG
1
;
Hui SUN
;
Rui AN
;
Xin-Hong WANG
;
Li-Sha YOU
;
Zheng-Yu CAI
Author Information
1. 上海中医药大学,上海201203
- Keywords:
Huxin Oral Liquid;
chemical constituents;
LC-MS/MS
- From:
Chinese Traditional Patent Medicine
2018;40(1):105-109
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous content determination of matrine,oxymatrine,salvia acid B,tanshinol,protocatechualdehyde,liquiritin,astragaloside,cinnamaldehyde,tanshinone Ⅱ A,ophiopogonin D and ruscogenin in Huxin Oral Liquid (Glycyrrhizae Radix et Rhizoma,Astragali Radix,Ophiopogonis Radix,etc.).METHODS The analysis of methanol extract of this drug was performed on a 25 ℃ thermostatic Ultimate XB-C18 column (4.6 mm × 100 mm,3 μm),with the mobile phase comprising of water-methanol (containing 0.05％ formic acid) flowing at 0.4 mL/min in a gradient elution manner.RESULTS Eleven constituents showed good linear relationships with-in their own ranges (r ＞0.999 0),whose average recoveries were 96.66％-103.2％ with the RSDs of 1.4％-3.4％.CONCLUSION This sensitive,reliable and accurate method can be used for the quality control of Huxin Oral Liquid.
