Simultaneous determination of thirteen constituents in Pientzehuang by UPLC-QQQ-MS
10.3969/j.issn.1001-1528.2018.01.019
- VernacularTitle:UPLC-QQQ-MS法同时测定片仔癀中13种成分
- Author:
Qiu-Mei HUANG
1
;
Yan-Ni TAI
;
Yan-Lin ZHU
;
Shao-Jun SHEN
;
Hui-Ting GUO
;
Ming-Qing HUANG
Author Information
1. 广东食品药品职业学院
- Keywords:
Pientzehuang;
chemical constituents;
UPLC-QQQ-MS
- From:
Chinese Traditional Patent Medicine
2018;40(1):101-105
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a ultra-high performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-QQQ-MS) method for the simultaneous content determination of notoginsenoside R1,ginsenoside Re,ginsenoside Rg1,ginsenoside Rb1,ginsenoside Rd,taurine,taurocholic acid,cholic acid,glycocholic acid,glycodeoxycholicacid,chenodeoxycholic acid,deoxycholic acid and muscone in Pientzehuang (Bovis Calculus,Moschus,Notoginseng Radix et Rhizoma,etc.).METHODS The analysis of methanol extract of this drug was performed on a 40 ℃ thermostatic Waters CORTECS UPLC C18column (100 mm × 2.1 mm,1.6 μm),with the mobile phase comprising of acetonitrile-water (containing 0.1％ formic acid) flowing at 0.25 mL/min in a gradient elution manner.RESULTS Thirteen constituents showed good linear relationships within their own ranges (r ＞0.998 5),whose average recoveries were 91.1％-105.3％ with the RSDs of 2.4％-4.6％.CONCLUSION This accurate,simple,sensitive and reproducible method can be used for the quality control of Pientzehuang.
